Our lab is deeply engaged in unraveling the functions of RNA-binding proteins, with a particular focus on Puf4 in the fission yeast Schizosaccharomyces pombe. RNA-binding proteins like Puf4 are critical in the post-transcriptional regulation of gene expression, influencing mRNA stability, localization, and translation. Puf4 belongs to the Pumilio and FBF (PUF) protein family, known for their conserved RNA-binding domains, which play key roles in controlling various developmental and cellular processes.

In S. pombe, Puf4 has been shown to regulate specific mRNAs by binding to their 3’ untranslated regions (UTRs). This interaction can lead to either the repression or stabilization of these mRNAs, depending on the cellular context. By modulating the fate of these mRNAs, Puf4 influences processes such as cell cycle progression, stress response, and differentiation.

Our research aims to dissect the precise mechanisms by which Puf4 exerts its regulatory functions. Through a combination of genetic, biochemical, and molecular biology approaches, we are identifying the target mRNAs of Puf4 and understanding how its activity is regulated within the cell. This work not only provides insights into the biology of S. pombe but also contributes to the broader understanding of RNA-binding proteins in eukaryotes.

Understanding Puf4’s role could have far-reaching implications, as RNA-binding proteins are often involved in human diseases, including cancer and neurodegenerative disorders. By studying Puf4 in a model organism like S. pombe, we hope to uncover fundamental principles of RNA regulation that are applicable across species.

Stay tuned as we continue to explore the fascinating world of RNA-binding proteins and their impact on cellular function!